Oct 3-5, 2022
Los Altos High School
LAHS Student Researcher at ASDRP
Reactivity informed design, synthesis, and targeted delivery of andrographolide and analogs, Nf-kB modulators for cancer treatment and degenerative diseases.
October 3, 2022 at 9:52:00 PM
Andrographolide, a labdane diterpenoid extracted from Andrographis paniculata, has been investigated as a therapeutic lead for many diseases. Namely, it has been found to exert its biological activity through inhibition of Nf-kB, a transcription factor at the crossroads of a myriad of cell signaling pathways, including those pertaining to tumor survival. Synthesis of derivatives of the natural product has the potential to create analogs and prodrugs of the compound that could improve tolerability, selectivity, and potency. Here we present the synthesis of C-ring modified andrographolide analogs with altered Michael acceptor properties, as well as a series of acid hydrolyzable acetal and ketal analogs, which we assay for controlled delivery and release. We use a quantitative reverse transcriptase PCR (qRT-PCR) assay as a proxy for Nf-kB regulated gene expression.
Meet the Speaker:
Keira is currently a senior at Los Altos High School as well as a student researcher at ASDRP. She started off in Edward Njoo's group focused on synthetic organic chemistry in 11th grade and has focused her efforts on the development of andrographolide and its analogs. Currently, her work at ASDRP investigates the physical properties of various close-in andrographolide analogs.